study on the interaction between isatin-β-thiosemicarbazone and calf thymus dna by spectroscopic techniques

نویسندگان

parvaneh pakravan young researchers and eliteclub, zanjan branch, islamic azad university, zanjan, iran

shahla masoudian department of chemistry, payame noor university, i.r. of iran

چکیده

the interaction between isatin-β-thiosemicarbazone (ibt) and calf thymus dna (ct-dna) was investigated in physiological buffer (ph 7.4) using neutral red (nr) dye as a spectral probe by uv–vis absorption and fluorescence spectroscopy, as well as viscosity measurements. the ibt is stabilized by intercalation in the dna ( k [ibt –dna] =1.03×105 m−1), and displaces the nr dye from the nr–dna complex. the binding constants kf and number of binding sites (n≈1) of ibt with dna were obtained by fluorescence quenching method at different temperatures. furthermore, the enthalpy and entropy of the reaction between ibt and ct-dna showed that the reaction is enthalpy-favored and entropy-disfavored. the changes in the base stacking of ct-dna upon the binding of ibt are reflected in the circular dichroic (cd) spectral studies. the viscosity increase of ct-dna solution is another evidence to indicate that, ibt is able to be intercalated in the dna base pairs.

برای دانلود باید عضویت طلایی داشته باشید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...

متن کامل

Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV–Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA ( K [IBT –DNA] =1.03×105 M−1), and displaces the NR dye from the NR–DNA comple...

متن کامل

Study on the Interaction between Isatin-β-Thiosemicarbazone and Calf Thymus DNA by Spectroscopic Techniques

The interaction between isatin-β-thiosemicarbazone (IBT) and calf thymus DNA (CT-DNA) was investigated in physiological buffer (pH 7.4) using Neutral Red (NR) dye as a spectral probe by UV-Vis absorption and fluorescence spectroscopy, as well as viscosity measurements. The IBT is stabilized by intercalation in the DNA (K [IBT -DNA] = 1.03×10(5) M(-1)), and displaces the NR dye from the NR-DNA co...

متن کامل

Spectroscopic study on the interaction of three water-soluble porphyrins with calf thymus DNA

Porophyrins and their metal derivatives are strong DNA binders with association constant of  to  . Some of these compounds have been used for the radiations sensitization therapy of cancer and are targeted to interact with cellular DNA. Binding of porphyrins to DNA changes the expectral and other physic chemical characteristic of porphyrins, The mode of binding can be extracted from inspection ...

متن کامل

Thermodynamic study of interaction between phthalocyanines with calf thymus DNA

The experimental determination of formation constant for interaction of two water soluble phthalocyanines,Cobalt (II) 4,4',4",4'"-tetrasulpho phthalocyanine (CoTsPc ) and Manganese(II) 4,4',4",4"-tetrasulphophthalocyanine (MnTsPc ) , with calf thymus DNA have been studied by UV-Vis spectrophotometric methodat lrnM phosphate buffer , pH 7.4 and at 5 different temperatures 20,25,30,35 and 400CC ....

متن کامل

Spectroscopic studies on the interaction of Fe3O4@CaAl LDH@Lamivudine with the calf thymus DNA

In this study, we were synthesized Fe3O4@LDH@Lamivudine and characterized by FT-IR spectroscopy,XRD and TEM. The interaction of this nanoparticle with CT-DNA was investigated by viscosity, circulardichroism (CD), Uv-Visible and fluorescence spectroscopy. Among all nanocarriers which applied as drugdelivery vectors, layered double hydroxides (LDHs) with exchangeable anions in t...

متن کامل

منابع من

با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید


عنوان ژورنال:
iranian journal of pharmaceutical research

جلد ۱۴، شماره ۱، صفحات ۱۱۱-۱۲۳

میزبانی شده توسط پلتفرم ابری doprax.com

copyright © 2015-2023